Figure 4

Representatice fluorescence photomicrographs of hAD specimens incubated in (A) Ringer´s solution at day 21 and (B) in bacteria mix (collagen autofluorescence excitation 488 nm, emission 514 nm). The figure shows a qualitative comparison of the matrix disaggregation incubated in control solution and bacteria mix. It can be seen, that matrix disaggregation in the bacteria group was more pronounced than in Ringer´s solution, presumably due to enzymatic cleavage (e.g. collagenases). Scale bars equal 50 μm.