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Two-trocar appendectomy in children – description of technique and comparison with conventional laparoscopic appendectomy
© The Author(s). 2016
Received: 31 March 2016
Accepted: 31 July 2016
Published: 4 August 2016
The aim of the study was to describe the technique of two-trocar laparoscopic appendectomy and compare the outcome between two- and three-trocar techniques in children.
All children who underwent laparoscopic surgery for suspected appendicitis from 2006 to 2014 in a center for pediatric surgery were included in the study. Converted surgeries and patients with appendiceal abscess or concomitant intestinal obstruction were excluded. A total of 259 children underwent appendectomy with either two (35 %) or three (65 %) laparoscopic trocars according to the surgeons’ preference and intraoperative judgment. Patient demographics, clinical symptoms, surgery characteristics, and complications were reviewed.
The mean age of the children was 10.4 years (range, 1–14 years). The mean follow-up time was 41.2 months (SD ± 29.2). No significant differences in age, gender, weight, or signs and symptoms were found between the two- and three-trocar groups. The mean surgery time was significantly shorter in the two-trocar group (47 min) than in the three-trocar group (66 min; p < 0.001). The rates of surgical complications were 2 % vs. 4 %, (p = 0.501), and the rates of postoperative complications were 0 % vs. 5 % (p = 0.054), in the two- and three-trocar groups. The overall incidence of postoperative wound infection was low (<1 %) and did not differ between groups.
Two-trocar laparoscopic appendectomy seems to be a safe and feasible technique with a low rate of postoperative wound infections. The present findings demonstrate that when the two-trocar technique could be applied, it is a good complement to the conventional three-trocar technique.
Appendicitis is the most common abdominal disease that requires surgery in children [1–5] . Most studies show that laparoscopic appendectomy (LA) has advantages over open appendectomy (OA) in children [6–8]. Publications also claim that intravenous antibiotics may serve as monotherapy for acute appendicitis [9, 10], but long-term results of these findings are lacking. Nonetheless, appendicitis is for sure, cured through appendectomy.
Since Kurt Semm described a technique for endoscopic appendectomy in 1983 , several surgical techniques for LA have been described. Currently, the technology for performing appendectomy utilizes one, two, three, or more trocars [11–14]. The two-trocar LA is a result of trying to overcome two major disadvantages of three-trocar LA when it originally was compared to OA; longer operative time and greater cost [15, 16]. Two-trocar laparoscopic-assisted appendectomy has been described previously in children but without comparison with other techniques [13, 16]. In adults, two-trocar LA has been compared with OA and conventional three-trocar LA [17–20]. In our pediatric surgery clinic, the laparoscopic technique utilizes two trocars or conventional LA with three trocars. Some surgeons are concerned about the two-trocar LA and afraid of increased rates of wound infections compared to conventional LA [16, 17, 20], and one study added cleansing of the wound with peroxide for 1 week postoperatively . Our hypothesis was that the two-trocar LA technique is as safe as three-trocar LA and does not increase the rate of wound infections. The aim of the present study was to describe the two-trocar LA technique in pediatric appendicitis and compare outcomes between two- and three-trocar techniques with regard to surgery time and complications, including the rate of postoperative wound infection.
The regional research ethics committee approved the study (registration no. 2010/49). Parents were informed about the intent to perform a LA and about the risk of conversion to open appendectomy. No specified information about the two- or three-trocar LA was given. Since this was a retrospective study of performed LAs, no written consent was taken beforehand.
Children and clinical data
All children (<15 years of age) who underwent LA from January 2006 to December 2014 in the Department of Pediatric Surgery were retrospectively included in the study. Exclusion criteria were converted LAs, patients with an appendiceal abscess, and patients with concomitant intestinal obstruction. Data were retrieved from an electronic database of medical records.
Acute appendicitis was diagnosed based on clinical prediction scores. The diagnosis was occasionally assisted by ultrasound. The diagnosis of appendicitis was confirmed by surgical findings combined with the histopathological analysis. Age, gender, weight, preoperative work-up, and appendiceal grade of inflammation was recorded. The time interval from admission to the start of surgery was defined as the time interval from the decision that the child should be transferred from the emergency room to the start of the operation. Information about the surgical method used (i.e., two- or three-trocar technique) and the duration of surgery were collected from the surgical reports. Postoperative pain medication was recorded from the moment the child left the postoperative unit and arrived in the pediatric surgical ward. Postoperative pain management, operative and postoperative complications (including wound infection), and duration of long-term follow-up were recorded.
Six surgeons attended and were responsible for the LAs. All of the responsible surgeons were specialists in general surgery or pediatric surgery. The decision to perform LA using the two-trocar technique was based on the surgeons’ intraoperative judgment for the individual child. In children where there was no need for diathermy or scissors to perform the appendectomy, only two trocars were used. All of the surgical interventions were preceded by antibiotic administration according to a previously published method . Postoperative antibiotics were given to patients with gangrenous or perforated appendicitis.
SPSS (Statistical Package for the Social Sciences) was used for the statistical calculations. A power calculation of the sample size was performed with the aid of a statistician . A minimum of 200 patients were needed to show a difference with 80 % power at a 5 % significance level. Children with appendiceal abscess were excluded to collect groups that are more comparable. To obtain comparable groups for calculation of pain management, patients with a negative appendectomy and patients with complications were excluded. Fisher’s exact test was used for dichotomous variables, and Student’s t-test and the Mann–Whitney U-test were used for ranked results with and without a standard distribution, respectively. Values of p < 0.05 were considered statistically significant.
The laparoscopy starts with the insertion of a 3- or 5-mm umbilical- or subumbilical trocar using an open access technique. It is intended for the insertion of 30-grade, 3- or 5-mm laparoscopic optics, which are used for diagnostic purposes. After having positioned the first trocar and collected some diagnostic information, the intraoperative findings enable the surgeon to choose in going on with two or three trocars.
If three-trocar LA is chosen, two 5-mm trocars is placed; one at 1–2 in. above the symphysis and one to the left (usually in the left iliac fossa). The dissection of the mesoappendix is performed using an electrocautery hook, and the appendix is divided at the base with staples.
Descriptive data on children with suspected appendicitis who underwent either two- or three-trocar laparoscopic appendectomy
Two-trocar LA (N = 91)
Three-trocar LA (N = 168)
10.3 ± 3.3
10.5 ± 2.8
Weight by age group (kg)
Time from admission to appendectomy (h)
Duration of symptoms (h)
CRP value (mg/L)
Fever > 38 °C
Grade of inflammation
Long-term follow-up (months)
47 ± 30
38 ± 28
With regard to the duration of symptoms, time to appendectomy, presence of fever or leukocytosis, and grade of inflammation, no significant differences were found between the two groups. However, a significantly higher rate of negative appendectomies was found in patients who underwent two-trocar LA (Table 1).
Evaluation of the surgical technique
The surgery time with the two-trocar technique was significantly shorter than with the three-trocar technique, both after inclusion and exclusion of negative appendectomies (Table 2).
Differences in surgery time and complications between two- and three-trocar laparoscopic appendectomies
Two-trocar LA (N = 91)
Three-trocar LA (N = 168)
Surgery time all included (min)
47 ± 16
66 ± 22
Surgery time with negative appendectomies and patients with surgical complications excluded (min)
46 ± 16
65 ± 20
Surgery time > 60 min
Surgery time in patients with surgical complications (min)
46 ± 1
81 ± 20
Number of surgical complications
Number of postoperative complications
Postoperative pain management
Two-trocar LA (N = 68)
Three-trocar LA (N = 135)
Total amount of morphinea (mg/kg)
0.11 ± 0.09
0.11 ± 0.09
Paracetamol intravenously (doses)
Advantages and disadvantages of two- vs. three-trocar laparoscopic appendectomy
• Less trauma
• Only two scars on the abdomen
• Shorter surgery time
• Shorter learning curve
• More instruments in the abdomen
• Can be used with adhesions or retrocecal appendix
• More often applicable
• Only one instrument
• Cannot use diathermy
• Limited mobility in the abdominal cavity and less able to explore the intestinal package
• Cannot get traction to resolve adhesions
• Not always applicable
• Longer surgery time
• More scars
• More trauma
Overview of studies of two-trocar LA in children and adults
Age group (N)
Valioulis et al. 
Umbilicus and pubic symphysis
Success: 76 %, mean operation time 19 min, wound infection 3 %.
Tekin and Kurtoglu 
Umbilicus and McBurney
Success: 67 %, mean operation time 46 min, wound infection 4 %.
Comparison with conventional LA.
Left iliac fossa and McBurney
Success 80 %, mean operation time 48 min, wound infection 11 %.
Malik et al. 
Comparison with OA
Umbilicus and McBurney
Success 11 %, mean operation time or wound infection not specified for two-trocar LA only.
Yagnik et al. 
Comparison with OA and conventional LA.
Umbilicus and Mcburney
Success 100 %, mean operation time 36 min, wound infection 1 %.
Baid et al. 
Comparison with conventional LA
Umbilicus and Mcburney
Success 84 %, mean operation time 24 min, wound infection 16 %
One fear surgeons have with the two-trocar technique is that it may result in a higher rate of wound infections, since the inflamed appendix comes in contact with all the layers of the abdominal wall and the skin when it is being drawn out [16, 17, 20]. However, with our technique, the outer part of the trocar enfolds the inflamed appendix, which prevents contact with the abdominal wall. Accordingly, the rate of wound infection in the present study was very low (1 %). Previously studies have reported rates between 1 and 16 % [13, 16, 17, 20, 21]. Diverse types of trocars may explain these differences.
The mean duration of two-trocar LA in the present study was similar to some reports [16, 17], but longer compared to others [13, 20, 21]. The shorter surgery time in the two-trocar group compared to the three-trocar group may be due to selection bias, hence the two groups were non-equivalent since the decision about which kind of procedure performed depended on the surgeon. We presume that each surgeon in this study used the surgical method that was the most beneficial for the children according to the own experience, and selected children for whom two-trocar LA could be applied.
Feasibility and advantages with the two-trocar LA
In the present study, none of the patients who initially underwent two-trocar LA were converted to three-trocar LA or open appendectomy. To a certain extent, this may be explained by selection bias mentioned above. In previous studies, the reported rate of successfully completed two-trocar surgeries was 11 % , 67 % , 76 % , 80 % , 84 %  and 100 % . From the present and previous studies, it is impossible to draw the conclusion that the shortened surgery time per se could be related to a lower complication rate. However, the two-trocar technique gives the surgeon more control over bleeding when dividing the mesoappendix. Also, less instruments are used and no staples are required, also reducing the cost. Since appendicitis is common, a small change in outcome can have major effect for the resources and costs of the health care system.
One limitation of the two-trocar technique is that it is difficult or impossible to use in a child with appendicitis and adhesions or retrocecal appendix . Further, it may not always be advisable to grasp and pull an inflamed appendix, which can be fragile and tortuous. This decision has to be based on the intraoperative judgment of the surgeon. Hence, if there is no need for diathermy or scissors to perform the appendectomy, a third trocar seems unnecessary. The technique described can be applied for fat patients with ample adipose tissue, but hardly for extremely fat patients. Nevertheless, the technique has been described in adults before in which the abdominal wall is much thicker compared to children. From this study, it seems that there may be three factors that influence which technique the surgeon chooses: 1) The anatomical position of the appendix. If the appendix has a distinct retrocecal direction, the two-trocar technique may be more difficult; 2) adhesions that fixate the appendix that only can be dissected with the help of cautery; and 3) the preference of the surgeon, hence experience with the method. Regarding the last factor, we consider the two-trocar LA, compared to conventional LA, to be easier to learn for the young surgeon. In summary, this leads us to conclude that because of assumedly shorter learning curve compared to single-port LA , faster surgery time , shortened anesthesia, less trauma and reduced costs compared to conventional LA , and being a safe procedure; two-trocar LA has a role among different laparoscopic techniques of appendectomy.
No definitive conclusions can be drawn regarding which of the two laparoscopic techniques is best, and under what circumstances each technique should be used, until a prospective, randomized study in children is conducted. However, the data presented in this report certainly add information that we can use in our daily practice. If we perform the two-trocar technique, it may benefit the child and the health care itself with reduced costs .
Two-trocar LA vs. single-port LA
Many studies have described the technique of single-incision LA (SILA) or single-port LA (SPLA). We agree that SILA/SPLA is interesting and we recently published an article about this technique . But, SILA/SPLA has also been shown to result in longer operative time, higher analgesic consumption, and greater hospital charges in children when a meta-analysis of RCT’s was performed . Another meta-analysis in children found shorter length of hospital stay, but higher conversion rate, higher surgical difficulty, and higher hospitalization costs compared with conventional LA . Quie et al. , concluded that “..there is no indication to use this approach over standard laparoscopic appendectomy”. Further, special instruments and longer learning curve are two other disadvantages of SPLA/SILA. Many appendectomies are performed by junior doctors/residents which have a harder time dealing with instrument collision, reduced operative work space, inadequate retraction and compromised view in SPLA/SILA . As stated in Table 4, one disadvantage of the two-trocar LA, is the reduced ability to explore the abdominal cavity, especially when examining the small intestine. However, this is also stated when talking about the SILA/SPLA . Together with the other advantages of the two-trocar LA mentioned before, we therefore believe there is an obvious role for the two-trocar LA among appendectomy techniques.
As mentioned above, the main weakness of the study is that the decision about which kind of procedure performed depended on the surgeon. Hence, an obvious selection bias may be evident and the two groups are non-equivalent. A second weakness of the study is that it was retrospective, and not randomized. When we now know that the two-trocar laparoscopic appendectomy does not result in more complications including wound infection, a prospective, randomized study can be started. At last, there was an unequal number of patients in the two groups compared. However, the only way this influences the statistical calculations is that the prerequisite to detect a, in beforehand given/hypothesized, difference is greater when the groups are equal in number (highest power). Hence, having different number of patients in the groups does not influence the statistical calculations and the conclusions drawn in this study.
Two-trocar LA seems to be a safe and feasible technique in children with a low rate of postoperative wound infections, and the present findings demonstrate that it could be considered as a good and safe complement to the conventional three-trocar technique. Future research will determine which method is the best treatment. Until then, the minimalized method described herein may be a good option.
LA, laparoscopic appendectomy; NSAID, non-steroidal anti-inflammatory drug; SD, standard deviation
The study was sponsored by grants from the Development Foundation of Region Skåne.
The study was sponsored by grants from the Development Foundation of Region Skåne.
Availability of data and materials
The database will not be shared due to restrictions formed by the ethical committee that approved this study.
MS collected the data, carried out the statistical analyses and drafted the initial manuscript. EJ collected the data and carried out parts of the statistical analyses. MH designed the figures and collected data. BO aided in the design of the study, controlled the statistical analyses and revised the manuscript. PS participated in the design of the study and revised the manuscript. EA designed the study, participated in the statistical analyses and revised the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests including commercial associations regarding the publication of this article.
Consent for publication
Since this was a retrospective study of performed LAs, no written consent was taken beforehand.
Ethics approval and consent to participate
The regional research ethics committee approved the study (registration no. 2010/49). Parents were informed about the intent to perform a LA and about the risk of conversion to open appendectomy. No specified information about the two- or three-trocar LA was given.
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- Addiss DG, Shaffer N, Fowler BS, Tauxe RV. The epidemiology of appendicitis and appendectomy in the United States. Am J Epidemiol. 1990;132(5):910–25.View ArticlePubMedGoogle Scholar
- Flum DR, Morris A, Koepsell T, Dellinger EP. Has misdiagnosis of appendicitis decreased over time? A population-based analysis. JAMA. 2001;286(14):1748–53.View ArticlePubMedGoogle Scholar
- Andersen S, Paerregaard A, Larsen K. Changes in the epidemiology of acute appendicitis and appendectomy in Danish children 1996–2004. Eur J Pediatr Surg. 2009;19(5):286–9.View ArticlePubMedGoogle Scholar
- Caperell K, Pitetti R, Cross KP. Race and acute abdominal pain in a pediatric emergency department. Pediatrics. 2013;131(6):1098–106.View ArticlePubMedGoogle Scholar
- Scholer SJ, Pituch K, Orr DP, Dittus RS. Clinical outcomes of children with acute abdominal pain. Pediatrics. 1996;98(4 Pt 1):680–5.PubMedGoogle Scholar
- Aziz O, Athanasiou T, Tekkis PP, Purkayastha S, Haddow J, Malinovski V, et al. Laparoscopic Versus Open Appendectomy in Children. Ann Surg. 2006;243(1):17–27.View ArticlePubMedPubMed CentralGoogle Scholar
- Sauerland S, Lefering R, Neugebauer EAM. Laparoscopic versus open surgery for suspected appendicitis. Cochrane Database Syst Rev. 2004;4:CD001546.Google Scholar
- Esposito C, Calvo AI, Castagnetti M, Alicchio F, Suarez C, Giurin I, et al. Open versus laparoscopic appendectomy in the pediatric population: a literature review and analysis of complications. J Laparoendosc Adv Surg Tech A. 2012;22(8):834–9.View ArticlePubMedGoogle Scholar
- Hartwich J, Luks FI, Watson-Smith D, Kurkchubasche AG, Muratore CS, Wills HE, et al. Nonoperative treatment of acute appendicitis in children: A feasibility study. J Pediatr Surg. 2015;51(1):111-16.Google Scholar
- Svensson JF, Patkova B, Almström M, Naji H, Hall NJ, Eaton S, et al. Nonoperative Treatment With Antibiotics Versus Surgery for Acute Nonperforated Appendicitis in Children. Ann Surg. 2015;261(1):67–71.View ArticlePubMedGoogle Scholar
- Kim HJ, Lee JI, Lee YS, Lee IK, Park JH, Lee SK, et al. Single-port transumbilical laparoscopic appendectomy: 43 consecutive cases. Surg Endosc Other Interv Tech. 2010;24(11):2765–9.View ArticleGoogle Scholar
- Gao J, Li P, Li Q, Tang D, Wang DR. Comparison between single-incision and conventional three-port laparoscopic appendectomy: A meta-analysis from eight RCTs. Int J Color Dis. 2013;28:1319–27.Google Scholar
- Valioulis I, Hameury F, Dahmani L, Levard G. Laparoscope-assisted appendectomy in children: the two-trocar technique. Eur J Pediatr Surg Off J Austrian Assoc Pediatr Surg [et al] = Zeitschrift fur Kinderchirurgie. 2001;11(6):391–4.Google Scholar
- Agresta F, De Simone P, Leone L, Arezzo A, Biondi A, Bottero L, et al. Laparoscopic appendectomy in Italy: an appraisal of 26,863 cases. J Laparoendosc Adv Surg Tech A. 2004;14(1):1–8.View ArticlePubMedGoogle Scholar
- Koontz CS, Smith LA, Burkholder HC, Higdon K, Aderhold R, Carr M. Video-assisted transumbilical appendectomy in children. J Pediatr Surg. 2006;41(4):710–2.View ArticlePubMedGoogle Scholar
- Tekin A, Kurtoglu HC. Video-assisted extracorporeal appendectomy. J Laparoendosc Adv Surg Tech A. 2002;12(1):57–60.View ArticlePubMedGoogle Scholar
- Konstadoulakis MM, Gomatos IP, Antonakis PT, Manouras A, Albanopoulos K, Nikiteas N, et al. Two-trocar laparoscopic-assisted appendectomy versus conventional laparoscopic appendectomy in patients with acute appendicitis. J Laparoendosc Adv Surg Tech A. 2006;16(1):27–32.View ArticlePubMedGoogle Scholar
- Malik AM, Talpur AH, Laghari A a. Video-assisted laparoscopic extracorporeal appendectomy versus open appendectomy. J Laparoendosc Adv Surg Tech A. 2009;19(3):355–9.View ArticlePubMedGoogle Scholar
- Yagnik VD, Rathod JB, Phatak AG. A retrospective study of two-port appendectomy and its comparison with open appendectomy and three-port appendectomy. Saudi J Gastroenterol. 2010;16(4):268–71.View ArticlePubMedPubMed CentralGoogle Scholar
- Baid M, Manoranjan K, Utpal D, Mukopadhyay M. Conventional Laparoscopic Appendicectomy and Laparoscope-Assisted Appendicectomy: a Comparative Study. Indian J Surg. 2015;77:330–5.View ArticlePubMedGoogle Scholar
- Yagnik VD, Rathod JB, Phatak AG. A retrospective study of two-port appendectomy and its comparison with open appendectomy and three-port appendectomy. Saudi J Gastroenterol [Internet]. 2010;16(4):268–71. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2995095&tool=pmcentrez&rendertype=abstract.View ArticleGoogle Scholar
- Nicola Z, Gabriella S, Alberto M, Saverio CF. Transumbilical laparoscopic-assisted appendectomy in children: Clinical and surgical outcomes. World J Gastrointest Endosc [Internet]. 2014;6(4):101–4. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3985149&tool=pmcentrez&rendertype=abstract.View ArticleGoogle Scholar
- Kulaylat AN, Podany AB, Hollenbeak CS, Santos MC, Rocourt DV. Transumbilical laparoscopic-assisted appendectomy is associated with lower costs compared to multiport laparoscopic appendectomy. J Pediatr Surg. 2014;49(10):1508–12.View ArticlePubMedGoogle Scholar
- Naredi M, Anderberg M, Stenström P, Arnbjörnsson E, Salö M. Single-Port Laparoscopy-Assisted Appendectomy in Children: A Method Described. J Surg. 2(3). http://0-dx.doi.org.brum.beds.ac.uk/10.16966/2470-0991.122.
- Ding J, Xia Y, Zhang ZM, Liao GQ, Pan Y, Liu S, Zhang Y, Yan Z. Single-incision versus conventional three-incision laparoscopic appendicectomy for appendicitis: a systematic review and meta-analysis. J Ped Surg. 2013;48(5):1088–98.View ArticleGoogle Scholar
- Qiu J, Yuan H, Chen S, He Z, Wu H. Single-port laparoscopic appendectomy versus conventional laparoscopic appendectomy: evidence from randomized controlled trials and nonrandomized comparative studies. Surg Laparosc Endosc Percutan Tech [Internet]. 2014;24(1):12–21. Available from: http://0-ovidsp.ovid.com.brum.beds.ac.uk/ovidweb.cgi?T=JS&PAGE=reference&D=medl&NEWS=N&AN=24487152.View ArticleGoogle Scholar
- St Peter SD, Adibe OO, Juang D, Sharp SW, Garey CL, Laituri CA, et al. Single incision versus standard 3-port laparoscopic appendectomy: a prospective randomized trial. Ann Surg. 2011;254(4):586–90.View ArticlePubMedGoogle Scholar